ARA Antibodies for Research Applications

Anti-human vimentin (clone V9)


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           "Binding of V9 monoclonal antibodies to human vimentin"


Hybridoma Clone    Antibody Isotype    Cat. No.
V9-2b                    Mouse IgG2b         ARA27

Source
A mouse was immunized with vimentin isolated from cow lens. Antibody-producing cells were isolated after fusing the spleen cells of the mouse with murine myeloma cells by selecting the cells that produced antibodies capable of staining mesenchymal cells in thin sections of human colon.

Specification:
The antibody does stain all cells that contain vimentin. Only frozen section can be used. The antibody only stains the 57kDa vimentin band in immunoblots performed on a lysate of human lymphocytes.

      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formalin embedded tissue) no
      Immunohistochemistry (antigen retieval) yes
      Flow cytometry yes
      Immunoblotting yes
      ELISA not tested
      RIA not tested
      Cross reactivity:
      Neurofilament not detected
      Glial fibrillar acidic protein not detected
      Desmin not detected
      Cytokeratin not detected
      Mouse vimentin not tested
      Rat vimentin not tested
      Rabbit vimentin not tested
      Goat vimentin not tested

        Immunoglobulin (sub)class:  IgG2b

        Presentation:  supernatant or protein A purified

        Storage:
        4C = 2 months
        -20C = 1 year
        Aliquoted storage at -70C or 20C is recommended

        Reference:
        1. Muijen GNP van (1984) Am J Pathol 116, 363-369.
        2. Muijen GNP van (1985) Hum Pathol 16, 590-595.
        3. Muijen GNP van (1987) Lab Invest 57, 359-369.
        4. Debus E et al. (1984) Eur J Cell Biol 34, 137-143.
        5. Muijen G van (1986) Exp Cell Res 162; 97-113
        6. Corver WE et al. (1994) Cytometry 15, 117-128
        7. Broek JCM van den et al. Appl Immunohis & Mol Morphology 8, 316-321, 2000

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