ARA Antibodies for Research Applications

Anti-human melanoma (clone PAL-M1)

           "Binding of PAL-M1 monoclonal antibodies to human melanoma"

Hybridoma Clone    Antibody Isotype    Cat. No.
PAL-M1                  Mouse IgG1          ARA35

A mouse was immunized with tissue derived from human melanoma metastases. After fusing the spleen cells from this mouse with myeloma cells, antibody-producing cells were screened on thin sections of various human tissues. Clone PAL-M1 was identified that produced antibodies staining malignant melanomas.

Antibody PAL-M1 stains the majority of primary melanomas and the large majority of metastases and is directed against the transferrin receptor (CD71). Local staining of dysplastic nevocellular nevi may be observed. Common nevocellular nevi rarely stain.

      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formalin embedded tissue) no
      Immunohistochemistry (antigen retrieval) no
      Flow cytometry yes
      Immunoblotting yes
      ELISA not tested
      RIA not tested
      Cross reactivity:
      Skin carcinoma not detectable
      Dendritic cells in inflammatory infiltrates present
      Sebaceous glands present
      Non-cutaneous tumor present
      Mouse melanoma not tested
      Rat melanoma not tested
      Rabbit melanoma not tested
      Goat melanoma not tested

        Immunoglobulin (sub)class: IgG1

        Presentation: supernatant 

        4C = 2 months
        -20C = 1 year
        Aliquoted storage at -70C or 20C is recommended

        1. Ruiter DJ et al., (1985) J Invest Dermatol 85, 4-8.
        2. Muijen G van et al. (1990) J Invest Dermatol 95, 65-69.
        3. Corver WE et al. (1994) Cytometry 19, 267-272.

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