Anti-human blood vessel endothelium (clone PAL-E (VC 3))
"Binding of VC3/PAL-E monoclonal antibodies to human blood vessel endothelium"
Hybridoma Clone Antibody Isotype Cat. No.
VC3/PAL-E Mouse IgG2a ARA39
Source
A mouse was immunized with human melanoma tissue. After fusing the spleen cells
from this mouse with myeloma cells, antibody-producing cells were screened on thin sections
of various human tissues. A clone was identified that produced antibodies reacting with the
endothelium of blood vessels.
Specification:
The antibody does stain human and various animal blood vessels with the exception of arteries.
| | Immunohistochemistry (frozen sections) | yes |
| | Immunohistochemistry (formalin embedded tissue) | no |
| | Immunohistochemistry (antigen retrieval) | no |
| | Flow cytometry | no |
| | Immunoblotting | not tested |
| | ELISA | not tested |
| | RIA | not tested |
| | Cross reactivity: |
| | Lymphatic endothelium | not detectable |
| | Sinus histiocytes | not detectable |
| | Megakaryocytes | not detectable |
| | Mouse endothelium | no |
| | Rat endothelium | no |
| | Rabbit endothelium | yes |
| | Goat endothelium | yes |
Immunoglobulin (sub)class: IgG2a
Presentation: supernatant
Storage:
4°C = 2 months
-20°C = 1 year
Aliquoted storage at -70°C or –20°C is recommended
Reference:
1. Schlingerman et al. (1987) Lab Invest 52, 71.
Ordering Information
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