ARA Antibodies for Research Applications

Anti-human blood vessel endothelium (clone PAL-E (VC 3))

           "Binding of VC3/PAL-E monoclonal antibodies to human blood vessel endothelium"

Hybridoma Clone    Antibody Isotype    Cat. No.
VC3/PAL-E              Mouse IgG2a         ARA39

A mouse was immunized with human melanoma tissue. After fusing the spleen cells from this mouse with myeloma cells, antibody-producing cells were screened on thin sections of various human tissues. A clone was identified that produced antibodies reacting with the endothelium of blood vessels.

The antibody does stain human and various animal blood vessels with the exception of arteries.

      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formalin embedded tissue) no
      Immunohistochemistry (antigen retrieval) no
      Flow cytometry no
      Immunoblotting not tested
      ELISA not tested
      RIA not tested
      Cross reactivity:
      Lymphatic endothelium not detectable
      Sinus histiocytes not detectable
      Megakaryocytes not detectable
      Mouse endothelium no
      Rat endothelium no
      Rabbit endothelium yes
      Goat endothelium yes

        Immunoglobulin (sub)class: IgG2a

        Presentation: supernatant 

        4C = 2 months
        -20C = 1 year
        Aliquoted storage at -70C or 20C is recommended

        1. Schlingerman et al. (1987) Lab Invest 52, 71.

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