Anti-human ovarian carcinoma (clone OV632)
"Binding of OV632 monoclonal antibodies to human ovarian carcinoma"
Hybridoma Clone Antibody Isotype Cat. No.
OV632 Mouse IgG2b ARA37
Source
A BALB/c mouse was immunized with cyst fluid of a human serous cystadenocarcinoma
of the ovary. After fusing the spleen cells from this mouse with murine myeloma cells,
antibody-producing cells were screened on thin sections of various human tissues.
A clone was identified that produced antibodies staining ovarian carcinomas.
Specification:
The antibody stains 80% of non-mucinous primary and metastatic ovarian cancers. This
monoclonal antibody is used for the identification of primary and metastatic non-mucinous
ovarian carcinoma and ovarian carcinoma cells in peritoneal fluids. It rarely stains
nongynaecological malignancies.
| | Immunohistochemistry (frozen sections) | yes |
| | Immunohistochemistry (formalin embedded tissue) | no |
| | Immunohistochemistry (antigen retrieval) | no |
| | Flow cytometry | yes |
| | Immunoblotting | no |
| | ELISA | not tested |
| | RIA | not tested |
| | Cross reactivity: | |
| | Breast carcinomas | not detectable |
| | Colon carcinomas | not detectable |
| | Other non-gynecological malignancies | incidentally present |
| | Other gynecological adenocarcinomas | present |
| | Mesothelial cells | not detectable |
| | Mouse ovarian tumors | not tested |
| | Rat ovarian tumors | not tested |
| | Rabbit ovarian tumors | not tested |
| | Goat ovarian tumors | not tested |
Immunoglobulin (sub)class: IgG2b
Presentation: supernatant or protein A purified
Storage:
4°C = 2 months
-20°C = 1 year
Aliquoted storage at -70°C or –20°C is recommended
Reference: 1. Fleuren GJ et al. (1987) Virchows Archiv A, 410, 481-486.
2. Boerman OC et al. (1991) Int J Gynecol 10, 15-23.
3. Delahye H et al., (1991) J Pathol 165 137-143.
4. Boerman OC et al. (1991) Anticancer Res 10, 1289-1296.
5. Corver WE (1995) Cytometry 19, 267-272.
Ordering Information
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