ARA Antibodies for Research Applications

Anti-human ovarian carcinoma (clone OV632)

           "Binding of OV632 monoclonal antibodies to human ovarian carcinoma"

Hybridoma Clone    Antibody Isotype    Cat. No.
OV632                   Mouse IgG2b         ARA37

A BALB/c mouse was immunized with cyst fluid of a human serous cystadenocarcinoma of the ovary. After fusing the spleen cells from this mouse with murine myeloma cells, antibody-producing cells were screened on thin sections of various human tissues. A clone was identified that produced antibodies staining ovarian carcinomas.

The antibody stains 80% of non-mucinous primary and metastatic ovarian cancers. This monoclonal antibody is used for the identification of primary and metastatic non-mucinous ovarian carcinoma and ovarian carcinoma cells in peritoneal fluids. It rarely stains nongynaecological malignancies.

      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formalin embedded tissue) no
      Immunohistochemistry (antigen retrieval) no
      Flow cytometry yes
      Immunoblotting no
      ELISA not tested
      RIA not tested
      Cross reactivity:
      Breast carcinomas not detectable
      Colon carcinomas not detectable
      Other non-gynecological malignancies incidentally present
      Other gynecological adenocarcinomas present
      Mesothelial cells not detectable
      Mouse ovarian tumors not tested
      Rat ovarian tumors not tested
      Rabbit ovarian tumors not tested
      Goat ovarian tumors not tested
        Immunoglobulin (sub)class: IgG2b

        Presentation: supernatant or protein A purified

        4C = 2 months
        -20C = 1 year
        Aliquoted storage at -70C or 20C is recommended

        Reference:        1. Fleuren GJ et al. (1987) Virchows Archiv A, 410, 481-486.   
        2. Boerman OC et al. (1991) Int J Gynecol 10, 15-23.
        3. Delahye H et al., (1991) J Pathol 165 137-143.
        4. Boerman OC et al. (1991) Anticancer Res 10, 1289-1296.
        5. Corver WE (1995) Cytometry 19, 267-272.

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