ARA Antibodies for Research Applications

Anti-human cytokeratin no.18. (clone M9)

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           "Binding of M9 monoclonal antibodies to human cytokeratin no. 18"

Hybridoma Clone    Antibody Isotype    Cat. No.
M9                        Mouse IgG1           ARA25

Source
A mouse was immunized with cytokeratin isolated from a human breast carcinoma cell line (MCF-7). Antibody producing cells were isolated - after fusing the spleen cells of this mouse with murine myeloma cells - by selecting the cells that produced antibodies capable of staining human simple epithelia in thin sections.

Specification:
The antibody does stain especially simple epithelia. Only frozen sections can be used in immunoperoxidase and immunofluorescence tests. In immunoblotting experiments the antibody was shown to recognize only keratin no.18 (45 kDa).

      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formal in embedded tissue) no
      Immunohistochemistry (antigen retrieval) yes
      Flow cytometry yes
      Immunoblotting yes
      ELISA not tested
      RIA not tested
      Cross reactivity:
      Other cytokeratins not detectable
      Vimentin not detectable
      Desmin not detectable
      Glial fibrillar acidic protein not detectable
      Neurofilament not detectable
      Mouse cytokeratin 18 not tested
      Rat cytokeratin 18 not tested
      Rabbit cytokeratin 18 not tested
      Goat cytokeratin 18 not tested
        Immunoglobulin (sub)class:  IgG1

        Presentation: supernatant 

        Storage:
        4C = 2 months
        -20C = 1 year
        Aliquoted storage at -70C or 20C is recommended.

        Reference:
        1. Muijen GNP van et al. (1987)  Exp Cell Res 171, 331-345.
        2. Dorst EBL et al. (1998) J Clin Pathol 51, 679-684.
        3. Corver WE et al. (1994) Cytometry 15, 117-128.

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