ARA Antibodies for Research Applications

Anti-human cytokeratin no. 8 (clone M20)

           "Binding of M20 monoclonal antibodies to human cytokeratin no. 8"

Hybridoma Clone    Antibody Isotype    Cat. No.
M20-2B                  Mouse IgG2b         ARA22

A mouse was immunized with cytokeratin isolated from a human breast carcinoma cell line (MCF-7). Antibody producing cells were isolated after fusing the spleen cells of this mouse with murine myeloma cells by selecting the cells that produced antibodies capable of staining human simple epithelia in thin sections.

The antibody stains almost all epithelial cells. In immunoblotting experiments the antibody was shown to recognize cytokeratin no. 8 (52 kDa).

      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formalin embedded tissue) no
      Immunohistochemistry (antigen retrieval) yes
      Flow cytometry yes
      Immunoblotting yes
      ELISA not tested
      RIA not tested
      Cross reactivity
      Other cytokeratins not detectable
      Vimentin not detectable
      Desmin not detectable
      Glial fibrillar acidic protein not detectable
      Neurofilament not detectable
      Mouse cytokeratin 8 not tested
      Rat cytokeratin 8 not tested
      Rabbit cytokeratin 8 not tested
      Goat cytokeratin 8 not tested
        Immunoglobulin (sub)class: IgG2b

        Presentation:  supernatant or protein A purified

        4C = 2 months
        -20C = 1 year
        Aliquoted storage at -70C or 20C is recommended

        1. Muijen GNP et al. (1987) Lab Invest 57, 359-369.
        2. Muijen GNP  et al. (1987) Exp Cell Res 171, 331-345.
        3. Dorst EBL et al. (1998) J Clin Pathol 51, 679-684.
        4. Corver WE et al. (1995) Cytometry 19, 267-272.

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