Anti-human lambda light chain (clone 48)
"Binding of clone 48 monoclonal antibodies to human lambda light chain"
Hybridoma Clone Antibody Isotype Cat. No.
Clone 48 Mouse IgG1 ARA43
Source
A mouse was immunized with a Bence Jones lambda protein. After fusing the spleen cells
of this mouse with mouse myeloma cells, antibody-producing clones were isolated and screened
for reaction with Bence Jones lambda protein from a second patient.
Specification:
The antibody will stain lambda chain-containing human cells. In ELISA the antibody will
react with free lambda chains and also with lambda chains attached to heavy chains.
Membrane bound Ig containing lambda light chains will also be recognized as well as
cytoplasmic Ig from bone marrow cells containing lambda chains. All cells reacting with
conventional anti-lambda will also react with antibody 48.
| | Immunohistochemistry (frozen sections) | yes |
| | Immunohistochemistry (formalin embedded tissue) | yes |
| | Immunohistochemistry (antigen retieval) | yes |
| | Flow cytometry | not tested |
| | Immunoblotting | yes |
| | ELISA | yes |
| | RIA | not tested |
| | Cross reactivity: |
| | Kappa | not detectable |
| | Mouse lambda | not tested |
| | Rat lambda | not tested |
| | Rabbit lambda | not tested |
| | Goat lambda | not tested |
Immunoglobulin (sub)class: IgG1
Presentation: supernatant or protein A purified
Storage:
4°C = 2 months
-20°C = 1 year
Aliquoted storage at -70°C or –20°C is recommended
Ordering Information
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