ARA Antibodies for Research Applications

Anti-human epidermal growth factor (EGF, clone E5)

Hybridoma Clone    Antibody Isotype    Cat. No.
EGF-E5                    Mouse IgG1           ARA40

A mouse was immunized with mouse EGF coupled to bovine serum albumin and to tetanus toxoid. After fusing the spleen cells from this mouse with murine myeloma cells, antibody-producing clones were isolated and screened for reaction with mouse EGF.

The antibody reacts with mouse and human EGF.

      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formalin embedded tissue) yes
      Immunohistochemistry (antigen retieval) no
      Flow cytometry not tested
      Immunoblotting yes
      ELISA yes
      RIA not tested
      Cross reactivity:
      Mouse EGF yes
      Rat EGF no
      Rabbit EGF not tested
      Goat EGF not tested
       Immunoglobulin (sub)class:   IgG1

       Presentation:   supernatant 

       4C = 2 months
       -20C = 1 year
       Aliquoted storage at -70C or 20C is recommended.
Reference: 1. Beerstecher HJ et al. (1988) J Histochem Cytohistochem 36, 1153-1160.

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