ARA Antibodies for Research Applications

Anti-human desmin (clone D33)

           "Binding of D33 monoclonal antibodies to human desmin"

Hybridoma Clone    Antibody Isotype    Cat. No.
D33                          Mouse IgG1        ARA18

A mouse was immunized with desmin isolated from human leiomyoma. Antibody producing cells were isolated, after fusing the spleen cells of this mouse with myeloma cells, capable of staining muscle cells in thin sections.

The antibody does stain muscle cells in human tissue. On immunoblots only the 52 kDa desmin band is stained (ref. 1).

      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formalin embedded tissue) yes
      Immunohistochemistry (antigen retieval) no
      Flow cytometry not tested
      Immunoblotting yes
      ELISA not tested
      RIA not tested
      Cross reactivity
      Glial fibrillar acidic protein not detectable
      Keratin not detectable
      Vimentin not detectable
      Neurofilament not detectable
      Mouse desmin not tested
      Rat desmin not tested
      Rabbit desmin not tested
      Goat desmin not tested
        Immunoglobulin (sub)class:  IgG1

        Presentation:  supernatant 

        4C = 2 months
        -20C = 1 year
        Aliquoted storage at -70C or 20C is recommended

        1. Muijen GNP van et al. (1987) Lab Invest 57, 359-369.
        2. Seidal T et al. (1987) Appl Pathol 5, 201-219.
        3. Ramaekers F et al. (1983) Histochemical J 15, 691-713.
        4. Broek JCM van et al. (2000) Appl Immunohis & Mol Morph 8, 316-321.

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