ARA Antibodies for Research Applications

Anti-human cytokeratin (clone 80)

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           "Binding of Clone 80 monoclonal antibodies to human cytokeratin"

Hybridoma Clone    Antibody Isotype    Cat. No.
Clone 80                  Mouse IgG1          ARA19

Source
A mouse was immunized with callus cytokeratins isolated from fresh human skin tissue. Antibody producing cells were isolated - after fusing the spleen cells of this mouse with murine myeloma cells by selecting the cells that produced antibody capable of staining human epithelia in thin sections.

Specification:
The antibody does stain all types of keratin containing (that is epithelial) cells in frozen sections of various tissues, with the exception of myo-epithelial cells and the podocytes of the glomeruli.


      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formalin embedded tissue) no
      Immunohistochemistry (antigen retrieval) yes
      Flow cytometry yes
      Immunoblotting yes
      ELISA not tested
      RIA not tested
      Cross reactivity
      Neurofilament not detectable
      Vimentin not detectable
      Glial fibrillar acidic protein not detectable
      Mouse cytokeratins yes
      Rat cytokeratins yes
      Rabbit cytokeratins not tested
      Goat cytokeratins not tested
        Immunoglobulin (sub)class:  IgG1

        Presentation:  supernatant  

        Storage:
        4C = 2 months
        -20C = 1 year
        Aliquoted storage at -70C or 20C is recommended

        Reference:
        1. Muijen GNP van (1984) Am J Pathol 114, 9.
        2. Muijen GNP van (1984) Am J. Pathol 116, 363.
        3. Muijen GNP van (1985) Hum Pathol 16, 590.
        4. Corver WE et al. (2000) Cytometry 41, 73-80.
        5. Broek, JCM van et al. (2000) Appl. Immunohis. & Mol. Morph. 8, 316-321.

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