Anti-human cytokeratin no. 4 (clone 6B10)
"Binding of 6B10 monoclonal antibodies to human cytokeratin no. 4"
Hybridoma Clone Antibody Isotype Cat. No.
Clone 6B10 mouse IgG1 ARA20
Source
A mouse was immunized with cytokeratin purified from human esophagus. Antibody producing cells
were isolated after fusing the spleen cells of this mouse with murine myeloma cells by selecting
the cells that produced antibodies capable of staining human epithelia in thin sections.
Specification:
The antibody stains non-cornifying squamous epithelium and transitional epithelium of the bladder,
cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands.
In immunoblotting experiments the antibody was shown to recognize only cytokeratin no.4 (59 kDa).
| | Immunohistochemistry (frozen sections) | yes |
| | Immunohistochemistry (formalin embedded tissue) | no |
| | Immunohistochemistry (antigen retrieval) | yes |
| | Flow cytometry | yes |
| | Immunoblotting | yes |
| | ELISA | not tested |
| | RIA | not tested |
|
| | Cross reactivity |
| | | Other cytokeratins | not detectable |
| | Vimentin | not detectable |
| | Desmin | not detectable |
| | Glial fibrillar acidic protein | not detectable |
| | Neurofilament | not detectable |
|
| | Mouse cytokeratin 4 | not tested |
| | Rat cytokeratin 4 | not tested |
| | Rabbit cytokeratin 4 | not tested |
| | Goat cytokeratin 4 | not tested |
Immunoglobulin (sub)class: IgG1
Presentation: supernatant or protein A purified
Storage:
4°C = 2 months
-20°C = 1 year
Aliquoted storage at -70°C or –20°C is recommended
Reference:
1.Muijen GNP et al. (1986) Exp Cell Res 162, 97-113.
2.Muijen GNP et al. (1987) Exp Cell Res 171, 331-345.
3.Dorst EBL et al. (1998) J Clin Pathol 51, 679-684.
4.Corver WE et al. (2000) Cytometry 39, 96-107.
Ordering Information
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