ARA Antibodies for Research Applications

Anti-human cytokeratin no. 4 (clone 6B10)

           "Binding of 6B10 monoclonal antibodies to human cytokeratin no. 4"

Hybridoma Clone    Antibody Isotype    Cat. No.
Clone 6B10             mouse IgG1            ARA20

A mouse was immunized with cytokeratin purified from human esophagus. Antibody producing cells were isolated after fusing the spleen cells of this mouse with murine myeloma cells by selecting the cells that produced antibodies capable of staining human epithelia in thin sections.

The antibody stains non-cornifying squamous epithelium and transitional epithelium of the bladder, cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands. In immunoblotting experiments the antibody was shown to recognize only cytokeratin no.4 (59 kDa).

      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formalin embedded tissue) no
      Immunohistochemistry (antigen retrieval) yes
      Flow cytometry yes
      Immunoblotting yes
      ELISA not tested
      RIA not tested
      Cross reactivity
      Other cytokeratins not detectable
      Vimentin not detectable
      Desmin not detectable
      Glial fibrillar acidic protein not detectable
      Neurofilament not detectable
      Mouse cytokeratin 4 not tested
      Rat cytokeratin 4 not tested
      Rabbit cytokeratin 4 not tested
      Goat cytokeratin 4 not tested
        Immunoglobulin (sub)class:  IgG1

        Presentation:  supernatant or protein A purified

        4C = 2 months
        -20C = 1 year
        Aliquoted storage at -70C or 20C is recommended

        1.Muijen GNP et al. (1986) Exp Cell Res 162, 97-113.
        2.Muijen GNP et al. (1987) Exp Cell Res 171, 331-345. 
        3.Dorst EBL et al. (1998) J Clin Pathol 51, 679-684.
        4.Corver WE et al. (2000) Cytometry 39, 96-107.

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