ARA Antibodies for Research Applications

Anti-human neurofilament (clone 2F11)

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           "Binding of 2F11 monoclonal antibodies to human neurofilament"

Hybridoma Clone    Antibody Isotype    Cat. No.
2F11                      Mouse IgG1            ARA28

Source
A mouse was immunized with human neurofilament isolated from the white matter from normal adult brain tissue. Antibody-producing cells were isolated, after fusing the spleen cells of this mouse with murine myeloma cells, by selecting the cells that produced appropriate antibodies as based on staining pattern in thin sections. Specification: The antibody does stain neurons in sections of brain and other tissues. On an immunoblot on a crude human brain extract the antibody will only stain the 70 kDa band of neurofilament (ref. 1)

      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formalin embedded tissue) yes
      Immunohistochemistry (antigen retieval) yes
      Flow cytometry not tested
      Immunoblotting yes
      ELISA not tested
      RIA not tested
      Cross reactivity
      Glial fibrillar acidic protein not detectable
      Cytokeratin not detectable
      Vimentin not detectable
      Mouse neurofilament not tested
      Rat neurofilament not tested
      Rabbit neurofilament not tested
      Goat neurofilament not tested
        Immunoglobulin (sub)class:  IgG1

        Presentation:  supernatant or protein A purified

        Storage:
        4C = 2 months
        -20C = 1 year
        Aliquoted storage at -70C or 20C is recommended

        Reference:
        1. Muijen GNP van et al. (1984) Am J Pathol. 116, 363-369
        2. Klck P et al. (1984) Lancet (March 24th), 652-653
        3. Muijen GNP van et al. (1985) Hum Pathol 16, 590-595
        4. Banks-Schlegel SP et al. (1985) Cancer Res 406, 339
        5. Hoefler H et al. (1985). Virch Arch 406, 339

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