ARA Antibodies for Research Applications

Anti-human cytokeratin no. 13 (clone 1C7)


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           "Binding of 1C7 monoclonal antibodies to human cytokeratin no. 13"

Hybridoma Clone    Antibody Isotype    Cat. No.
Clone 1C7                Mouse IgG2a         ARA23

Source
A mouse was immunized with cytokeratin purified from human esophagus. Antibody producing cells were isolated after fusing the spleen cells of this mouse with murine myeloma cells by selecting the cells that produced antibodies capable of staining human epithelia in thin sections.

Specification:
The antibody does not stain non-cornified squamous epithelia, except cornea and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia (ref. 1). In immunoblotting experiments the antibody was shown to recognize only cytokeratin no. 13 (54 kDa).

      Immunohistochemistry (frozen sections) yes
      Immunohistochemistry (formalin embedded tissue) no
      Immunohistochemistry (antigen retrieval) yes
      Flow cytometry not tested
      Immunoblotting yes
      ELISA not tested
      RIA not tested
      Cross reactivity
      Other cytokeratins not detectable
      Vimentin not detectable
      Desmin not detectable
      Glial fibrillar acidic protein not detectable
      Neurofilament not detectable
      Mouse cytokeratin 13 not tested
      Rat cytokeratin 13 not tested
      Rabbit cytokeratin 13 not tested
      Goat cytokeratin 13 not tested
        Immunoglobulin (sub)class: IgG2a

        Presentation: supernatant or protein A purified

        Storage:
        4C = 2 months
        -20C = 1 year
        Aliquoted storage at -70C or 20C is recommended

        Reference:
        1. Muijen GNP et al. (1986) Exp Cell Res 162, 97-113.
        2. Muijen GNP  et al. (1987) Exp Cell Res 171, 331-345.
        3. Dorst EBL et al. (1998) J Clin Pathol 51, 679-684.
        4. Corver WE et al. (1994) Cytometry 15, 117-128.

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