Monoclonal antibodies directed against rat CD8


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                "Binding of OX8 (IgG2a) monoclonal antibodies to CD8-positive
                T cells or NK cells in freshly frozen rat liver tissue"

Hybridoma Clone    Antibody Isotype    Cat. No.
OX8-2a                     Mouse IgG2a         ARA03
OX8-2b                     Mouse IgG2b         ARA04

Generation
  These two monoclonal antibodies are IgG2a and IgG2b isotype switch variants 
  (Kuppen et al., 1993) of the well-known OX8 IgG1 antibody that is specific for rat CD8 
  (Brideau et al., 1980).
Reactivity 
  Both monoclonal antibodies bind to  a  rat  strain-independent epitope of the a-chain 
  of CD8, present on the CD8-positive subsets of T cells and NK cells.
Technical information 
  The OX8 switch variants are useful in flow cytometric analysis of rat lymphocytes, 
  especially in multicolor stainings. The OX8 switch variants can also be used in 
  immunohistochemistry, both on fresh frozen tissue and formalin-fixed/paraffin-embedded 
  tissue. Citric acid pretreatment is recommended for formalin-fixed/paraffin-embedded tissue 
  sections.
References 
  Kuppen PJ, Eggermont AM, Smits KM, Van Eendenburg JD, Lazeroms SP, Van De Velde CJ,
  Fleuren GJ (1993) The development and purification of a bispecific antibody for lymphokine-
  activated killer cell targeting against the rat colon carcinoma CC531. Cancer Immunol 
  Immunother 36: 403-408

  Brideau RJ, Carter PB, McMaster WR, Mason DW, Williams AF (1980) Two subsets of rat 
  T lymphocytes defined with monoclonal antibodies. Eur J Immunol 10: 609-615.
Ordering Information